Part:BBa_K3058002

Uric acid-induced Uricase generator

This device could produce uricase only when there is uric acid. In the device, HucR is a repressor than can bind to HucO operator to inhibit the gene expression down stream HucO. In the presence of uric acid, the structure of HucR is altered and is no longer able to bind HucO, leading to the down stream gene expression. We inserted a uricase gene (URI) downstram HucO. In this case, uricase is expressed only when there is significant level of uric acid. To better visualize the gene expression, we include RFP(E1010) downstream HucR and a GFP(E0040) downstream URI. In our project, we express it in E. coli. It is hoped that the uricase-expressing E.coli can lower the uric acid concentration in human alimentary canal, so as to prevent gout disease.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 2832
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2362
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 2832
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 2832
  Illegal NgoMIV site found at 867
  Illegal AgeI site found at 156
  Illegal AgeI site found at 268
  Illegal AgeI site found at 2384
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 3498